This e-book was created as a result of the collaboration of partner universities within the V4 project, supported by the Visegrad Fund (project number 22410207). Its goal is to support the implementation of innovative solutions using artificial intelligence (AI) in higher education. AI is an increasingly important phenomenon that brings new challenges and opportunities, especially in digitalizing education, developing digital literacy, and preparing students for the future labour market. However, academic staff and students often lack sufficient information and skills regarding the appropriate and ethical use of AI. The e-book is structured to inspire academic staff to apply AI in various areas of teaching and research. It contains examples from the actual practice of universities from three countries that contributed examples to the e-book: the Czech Republic, Poland, and Hungary. These examples illustrate the transformation of study programmes, new teaching and assessment methods, the use of modern AI tools, building AI-related competencies, and ensuring responsible and ethical approaches to this technology. It is hoped that this publication will help broaden awareness of AI possibilities, and support innovation in higher education.